Fluorescence is the most versatile detection technique currently available for biomedical research. No other technique can be used to address as many different types of samples using as many different instrumentation platforms. This volume describes the technical basis for this versatility and provides a complete look at the wide span of resulting applications. The first part of the text examines basic photophysics of the fluorescence process, explains the properties of fluorescent molecules, and presents instrumentation platforms. Organized by classes of biological molecules, the second part of the text focuses on applications.
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